Assessment of methicillin resistant Aureus detection methods: analytical comparative study.

Ibrahim, Omer Mohammed Ali and Bilal, Naser Eldin and Osman, Omran Fadl and Magzoub, Magzoub Abbas (2017) Assessment of methicillin resistant Aureus detection methods: analytical comparative study. The Pan African medical journal, 27. p. 281. ISSN 1937-8688

Full text not available from this repository.

Abstract

Introduction The heterogeneous expression of methicillin resistance in (MRSA) affects the efficiency of tests available to detect it. The objective of this study was to assess four phenotypic tests used to detect MRSA. Methods This is an analytical comparative study conducted among sudanese patients during period from May 2012 to July 2014, strains were isolated and identified by conventional methods, and then confirmed by PCR detection of coagulase gene. PCR detection of mecA gene was used as a gold standard to assess oxacillin resistance screen agar base (ORSAB), oxacillin disc, cefoxitin disc (at different temperatures and incubation periods) and MRSA-latex agglutination test. ATCC 25923 was used as control. Sensitivity and specificity were calculated. Results MRSA- latex agglutination was the most accurate test; it showed 100% of both sensitivity and specificity, followed by cefoxitin disc with sensitivity of 98.48% and specificity of 100%. However, both of oxacillin disc and oxacillin resistance screen agar base showed less accurate results, and were affected by incubation periods. Oxacillin disc after 24 h incubation both at 30°C and 35°C showed sensitivity and specificity values of 87.88% and 96.23%, respectively. However, after 48h incubation the test at 30°C showed sensitivity and specificity values of 89.39%, and 94.34%, respectively. At 35°C (48h) it showed values of 89.39%, 92.45% respectively. Specificity of ORSAB was more than oxacillin disc at 35°C after 24h incubation 98.11% and 96.23%, respectively. Conclusion MRSA- latex agglutination and cefoxitin disc diffusion tests are recommended for routine detection of MRSA.

Item Type: Article
Subjects: Q Science > QR Microbiology
Divisions: University of Khartoum > Faculty of Science > Department of Biology
Depositing User: Unnamed user with email almegdadsharaf@gmail.com
Date Deposited: 30 Jul 2018 13:16
Last Modified: 30 Jul 2018 13:16
URI: http://search.srh.edu.sd/id/eprint/4933

Actions (login required)

View Item View Item